| Catalog number: |
PPL50616-3 |
| Plasmid name: |
pSpCas9(BB)-2A-GFP-mRb1 sgRNA (3+1) |
| Quantity: |
Kit |
| Gene/insert name: |
Rb1 sgRNA |
| Alt name: |
p110-RB1; pp105; pRb; Rb; Rb-1 |
| Insert size: |
20bp |
| Species: |
Mus musculus |
| Gene ID: |
19645 点击查看NCBI相关信息 |
| Accessions: |
N/A 点击查看NCBI相关信息 |
| Vector Backbone: |
pSpCas9(BB)-2A-GFP (PX458) |
| Tag: |
3*Flag(N-term);2A-EGFP(C-term) |
| Backbone manufacturer: |
N/A |
| Vector type: |
CRISPR |
| Backbone size w/o insert: |
9288bp |
| Cloning site 5': |
Bbs I |
| Site destroyed during cloning: |
Yes |
| Cloning site 3': |
Bbs I |
| Site destroyed during cloning: |
Yes |
| Bacterial resistance(s): |
Ampicillin |
| Growth strain(s): |
Top10 |
| Growth temperature: |
37 |
| High or low copy: |
High Copy |
| Plasmid description: |
CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting Mus musculus RB transcriptional corepressor 1 (Rb1) |
| Selectable markers: |
None |
| Map: |
PPL50616-3a |
| 产品分类: |
CRISPR |
cDNA克隆
pSpCas9(BB)-2A-GFP-mRb1 sgRNA (3+1)
